Purification of Hepoxilin Epoxide Hydrolase from Rat Liver
نویسندگان
چکیده
منابع مشابه
Mammalian soluble epoxide hydrolase is identical to liver hepoxilin hydrolase.
Hepoxilins are lipid signaling molecules derived from arachidonic acid through the 12-lipoxygenase pathway. These trans-epoxy hydroxy eicosanoids play a role in a variety of physiological processes, including inflammation, neurotransmission, and formation of skin barrier function. Mammalian hepoxilin hydrolase, partly purified from rat liver, has earlier been reported to degrade hepoxilins to t...
متن کاملSoluble epoxide hydrolase in rat inflammatory cells is indistinguishable from soluble epoxide hydrolase in rat liver.
Soluble epoxide hydrolase (sEH) is a ubiquitous mammalian enzyme for which liver and kidney are reported to have the highest activity. We have shown that the soluble epoxide hydrolase (sEH) activity present in rat neutrophils and macrophages is kinetically, immunologically, and physically indistinguishable from rat liver cytosolic sEH. Cytosol from rat liver or inflammatory cells and recombinan...
متن کاملPurification and identification of an epoxide hydrolase from equine liver.
In the horse, drug metabolism has been shown to be complex, but little is known about the proteins involved in these processes [l]. As a result of work on the purification of equine Cytochrome P450 enzymes, an epoxide hydrolase has been purified [2]. Epoxide hydrolases catalyse the hydration of biologically active epoxides formed during the metabolism of many drugs and mutagenic compounds [3]. ...
متن کاملAffinity purification of cytosolic epoxide hydrolase from human, rhesus monkey, baboon, rabbit, rat and mouse liver.
An affinity purification system based on elution of cytosolic epoxide hydrolase from a methoxycitronellyl thiol ligand with 4-azidochalcone oxide was applied to a variety of samples including liver from human, monkey, baboon, rabbit, rat and mouse as well as mammary gland from mouse. Hepatic tissues yielded a major 58 kDa band on SDS-PAGE, but the system had to be modified slightly to remove a ...
متن کاملMicrosomal epoxide hydrolase of rat liver. Purification and characterization of enzyme fractions with different chromatographic characteristics.
Microsomal epoxide hydrolase was purified from rat liver, and different fractions of the purified enzyme, which varied in their contents of phospholipid, were obtained by ion-exchange chromatography. One fraction (A), which did not bind to CM-cellulose, had a high phospholipid content, and a second fraction (B), which was eluted from CM-cellulose at high ionic strength, had a low phospholipid c...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1989
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)60532-0